health advisory >> medicine >> why we can not use other enzymes in ELISA technique except HRP,AP etc

why we can not use other enzymes in ELISA technique except HRP,AP etc

health advisory Hospital Doctor Yu 2007-11-8 19:09:36

ELISA is technique used in idenification of endemic region for perticular diseases ,its interaction of antigens and antibodies

Answers:

The enzyme is used to quantify the reaction, The enzyme HRP is attached to the secondary antibody and is therfore able to change the colour of the substrate from clear to a coloured solution. Which can then be measured for absorbance. The enzymes used are most likely because they are common enzymes that are known to be reliable and cheap to attach to the secondary antibody.


Why was the biochemical evouti..
Why was I born
why vitamin a important to bod..
Why use embryos for stem cell ..
Why truck drivers and secretar..
Why to take Albendazole or Meb..
why to favor embryonic stem ce..
Why thermometers are kept
Why RNA seperation only view 2..
why respiratory alkalosis do n..
health advisorysitemaps.xml